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KMID : 0857220040080020379
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Journal of the Korean Pediatric Cardiology Society
2004 Volume.8 No. 2 p.379 ~ p.386
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Expression of Mitochondrial Gene by Using cDNA Microarray System in Adriamycin Treated Rat Heart
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Hong Young-Mi
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Abstract
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Purpose: cDNA microarray system(DNA chip) can permit rapid and accurate diagnostic screening of multiple DNA. It helps to obtain the gene expression profile, identify rapidly and validate disease specific biomarkers. The purposes of this study were to investigate the decreased gene expression after administration of adriamycin and make a guidance for gene therapy in adriamycin induced cardiac injury model.
Methods: Sprague-Dawley rats were divided into two groups. The first group was the control. The second group was given adriamycin(5 mg/kg), twice a week over a period of two weeks. Rats were sacrified after 2 weeks. Rat heart was quickly dissected and frozen with liquid nitrogen. Rat’s tissues for RNA extraction were stored in -70℃. cDNA microarray analysis was performed by using a set of 5,184 sequence-verified rat cDNA clones. Fluorescent cDNA probes hybridized to a cDNA microarray were detected by scanning the slide with a GenePix 4000B scanner. Specific decreased genes were verified by reverse transcription polymerase chain reaction(RT-PCR).
Results: Rat electron transfer flavoprotein(RETF), cytochrome c and Rat F-0- ATPase subunit mRNA were depressed after treatment of adriamycin.
Conclusion: Rapid screening of multiple decreased gene expression by DNA chip is useful diagnostic test to detect early cardiac injury before developing adriamycin induced cardiomyopathy. It may offer optimizing adriamycin dose, and it will be the basis of gene therapy for cardiomyopathy in the future.
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KEYWORD
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Gene expression, Mitochondria, Adriamycin
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